Introduction: The aim of this study was to investigate root canal morphology of mandibular anterior teeth using cone beam computerized tomography (CBCT) in Saudi subpopulation. Materials and methods: A total of 298 patients who had examined with CBCT. Including 1,788 mandibular anterior teeth. The examinations were done by using CBCT are number of roots, number of root canals and canal configuration according to Vertucci’s classification. Result: Among demographic variables, there were 120 males and 178 female, with maximum of age 68 and minimum of age 12. All the incisors show single root. Type I was the most significant type among the examined teeth (86.9%, n=1554). The prevalence of two root canals in lateral incisors (20.3%, n=121) found to be higher than central incisor (16.8% n=100). The major pattern of two root canals system was type II in mandibular incisor (74.9%, n=143), the incidence of other types (III, IV, V and VI) was (37.2%, n=111). According to the result, the presence of two roots in mandibular canines was (0.9%, n=3). Type I pattern was highly prevalent (97.85%, n=583) and two root canals system (2.2%, n=13) Conclusion: Two root canals in mandibular anterior teeth have been found in current study with percentage of (13.1%), and type II was the most predominant type in two root canals types.