A New Technology for the Synthesis of Long DNA Strings
Jaroslav Pavelka* ,Tereza Kozelkova
We present a new theoretical technical solution application of nanopore sequencing (Oxford Nanopore Technologies, https://www. nanoporetech.com/), which has primarily been used to read molecules. We describe how to apply nanopore sequencing for the production of a new type of device synthesing of molecules according to our requirements of a chosen database that has been prepared in advance. This technology can implement the programmed synthesis of long DNA strings, ideally entire chromosomes. We propose a device using nanopores and a system of nanotubes that can synthesize molecules using individual nucleotides, existing single-stranded DNA (ssDNA) ligase or modified forms of ssDNA ligase and the energy provided by ATP. The second strand is synthesized by Taq polymerase, which is located below the nanopore. The synthesis of long strings is performed using many nanopores positioned linearly. The strings are collected in wells below the nanopore, after which the strings are ligated to form a long single string (or to form part or all of a chromosome) by double-stranded DNA (dsDNA) ligase. There are many options for successful implementation, particularly in the field of genetic engineering.
