Tumor Treatment by A Combination of Chemotherapy and Radiotherapy with Methotrexate Drug
Sahar E. Abo-Neima*, Abdalah Y. Atia, Hussein A. Motaweh
Many ways investigated for cancer treatment, including surgery, chemotherapy, and radiotherapy .In our study new method was performed as photodynamic laser therapies with nanoparticles (NPs), Methotrexate drug (MTX), direct electric current (DEC) and combinations of them which give an interesting combined therapeutic effect. The Primary Purpose of this work was to investigate the ability of NPs to transform laser energy and DEC into therapeutic heating. Characterization of prepares NPs, tumor weight, volume, and histopathology investigated. The results showed that the tumor capsule infiltrate by inflammatory cells. Mice treated with NPs and MTX shows a similar degree of infiltration areas of necrosis and newly formed blood capillaries are also noticeably. Mice treated with NPs, MTX and low-level laser therapy (LLT) shows increased tumor cell necrosis with aggregates and sheets of invasive malignant cells. Mice treated with NPs, MTX and DEC shows slightly increased tumor cell necrosis and increased mononuclear inflammatory cells. Mice treated with NPs, MTX, LLT and DEC shows massive tumor cell necrosis with only minimal aggregates of malignant cells. It concluded that treat tumor with LLT, DEC, MXT, and NPs give us the most inhibition for it.