In Vitro Regeneration of Jasminum Azoricum L : An Important Ornamental and Medicinal Plant
Siham Abd Alrazzaq Salim*
This study was conducted in order to investigate the ability of Jasminum azoricum L. to regenerate in vitro through two waiys; in the first way: nodal explants were cultured on MS medium containing different concentrations of BA ( 0.0 , 0.5 , 1.0 , 2.0 , 4.0 , or 6.0 ) mg/L for direct shoot proliferation. The results showed that BA in concentration 2.0 mg/L gave the best result in shoot number( 2.5 ), while the control was the best in shoot length ( 2.51 cm ) and number of nodes per shoot( 4.5 node / shoot ) than other treatments. In the second method: internodal explants were cultured on MS containing BA( 0.0 , 2.0 , 4.0 , or 6.0 ) mg/L with 2,4-D ( 0.0, 0.01 , 0.05 or 0.1) mg/L for callus induction and indirect regeneration of shoots. The highest percent of callus induction ( 100% ) was seen in MS supplemented with 4.0 mg/L BA + 0.1 mg/L 2,4-D and 6.0 mg/L BA + 0.1 mg/L 2,4-D. The proliferated callus was transferred into MS medium supplemented with BA ( 0.0 , 2.0 , 4.0 , or 6.0 ) mg/L with Kin ( 0.0 or 2.0) mg/L for adventitious buds regeneration. The highest number of buds (10.1) was seen in the combination 4.0 mg/L BA + 2.0 mg/L Kin. In vitro rooting was achieved in medium containing 1.0 mg/L IBA and the rooted plantlets were successfully acclimatized.
