Vijay Kumar, Narotam Sharma, Dalel Singh, Satish Chandra Nautiyal, Baljeet Singh

 

The distribution of HCV genotypes vary according to the geographical region. Genotypes 1-3 are widely distributed throughout the world. The outcome of HCV genotyping is of almost clinical value. Current research work was carried out to study the Cellular and Molecular profiling of Hepatitis C Virus (HCV) and its clinical importance that includes the correlation of the different laboratory parameters with

respect to HCV RNA Viral load and its genotypes. 94 EDTA blood samples were collected from HCV reactive patients and further processed for cellular and molecular profiling. Out of 94 HCV RNA quantified, 42 (44.6%) were TND, 52(55.4%) with HCV RNA viral load >34 IU/ml. HCV genotype 3 came in 21 (65.62%) cases followed by HCV genotype 1a with 4 (12.5%) and 6 with 3(9.37%) and 1b with 2 with 6.25%. SGOT, SGPT, Alkaline phosphatase, Bilirubin and Globulin was elevated in 28 (62%), 22(48.88%), 23 (51.11%), 12(26.66%) and 20(44.44%) respectively. Maximum number of cases with HCV RNA Viral load was found in the 1.00 X 104 -1.00 X 107 IU/ml range with 27 cases. The HCV genotype 3 is one of the most replicating virus known to damage hepatic cells & thus requires proper line of treatment thoroughly during the diagnosis. Thus, the area of molecular testing for the diagnosis and management of HCV infection has shown steady improvement in technology and standardization. Further to say, development of proper algorithm by combining the profiling of cellular, molecular and Biochemical studies can be a new era for the proper management of this virus.

Tuberculosis is major threat in today’s society. Also, there is rapid increase in multi-drug resistant cases so there is urgent requirement of novel therapeutic drug target. Several molecules are present in mycobacterial cell that are crucial in host pathogen interaction thus recognition and simultaneous expression of such molecules might show some upregulation or downregulation of those molecules inside host. Understanding of whole signalling cascade involved behind receptor-molecule interactions would be key in finding some novel targets for drug designing. Recently, many promising target receptors like Toll like receptor (TLR-2 and 4), G-protein coupled receptor (GPCRs); adhesion GPCRs (aGPCRs), CXCR1, CXCR2, etc and molecules like GTPases; Calcium (Ca2+); PknA, PknB, PknG, NAD kinase, NAD synthetase; etc, are likely to be studied that can be used as target but yet proper mechanistic understanding of these receptor : pathogen interactions are obscure. G-proteins are important class of protein present in eukaryotes. But in prokaryotes these proteins had remain long ignored however in recent time targeting these G-proteins in prokaryotes is a major interest in research and detailed study could be used as a drug target which could be a promising diagnostic approach. In our study we have shown that conserved GTP binding protein like era, obg, elongation factor, engA, ftsY, etc consists of GTP binding or hydrolysing property and thus disruption of these genes is directly coefficient to the survival and pathogenecity of the organism. So we hypothesize that if we interrupt or manipulate binding of these genes with the receptor present in eukaryotes via use of some manipulative signal transduction then it could be

beneficial for check of this disease.