Determination of Oxide and Reducing Glutathione Levels by Glutathione Peroxidase Activity in Stomach Cancer Patients
Kemal Gokce, Seker Dag*
In this study, glutathione peroxidase activity (GPx) (EC:1.11.1.19) and amount of reduced glutathione (GSH) and oxidized glutathione disulfite was investigated in blood samples of who diagnosed with colon cancer individuals whose ages between 18-75. Erythrocyte were isolated immediately from patient and control groups after 3 ml of blood samples were taken. Amount of hemoglobin was determined in presence of cynanide with conversion of methemoglobin to cynomethemglobin at 540 nm by standart curve. GPx activity was determined by oxidation of NADPH at 340 nm spectrophotometrically. Amounts of GSH and GSSG were determined at 412 nm by using standard curve.
While GPx activity of stomach cancer patients (5,80 ± 1,40 U/gHb), contcentration of GSH (7,66 ± 1,19 nmol/gHb) and GSH/GSSG ratio (1,29 ± 0,46) significantly decreased comparing to control group (GPx: 10,52 ± 2,22 U/gHb, GSH: 11,43 ± 1,90 nmol/gHb, GSH/ GSSG: 3,86 ± 1,30), amount of GSSG of patient group (6,33 ± 1,18 nmol/gHb) significantly increased comparing to control group (3,09 ± 0,48 nmol/gHb). These results suggested that antioxidant/oxidant system balance abolished in patients who has stomach cancer.
