This research aims to create a peak run-off mode based on land use existing and masterplan Sentul City, an urban area. To determine the peak run-off by rational method, the study uses the formulation as follows: Q = 0.2778.C.I.A, in which Q is the peak run-off, C is the run-off coefficient of area, I is the average rain rate intensity, and A is the area of study. For recognizing the existing of land use, the researcher used image analysis SPOT-6 (2017) by supervised classification. It estimated gamma distribution parameter through maximum likelihood method by using software QGIS 2.8, SAGA GIS, dan Arc-GIS 10.4.1. According to the analysis, the study result shows the land use existing peak run-off coefficient value and masterplan are 0.40 and 0.61, respectively, in which the difference is 0.21. The peak run-off increase is 25.32 m3/second due to changes in land use, so it must be anticipated so as not to negatively impact the environment, social, and economy such as: aggravating and expanding flooding areas, causing water shortages in the dry season, increasing erosion and sedimentation, etc.

Kary Banks Mullis in 1984 was the scientist who invented the technique of polymerase chain reaction (PCR), this technique further modified into real-time PCR. The specific role of Real-Time PCR in observing and expressing genes in real time has altered the scientific era of 21st century in especially life sciences. Its excellent role in quantitative traits measurement, genetic potential variability in intra and inter organisms, in the early hours diagnosis of disorders, forensic fields and many others has revolutionized the scientific research. Here, we will briefly discuss various aspects of real-time PCR, including its technique, applications and challenges in all scientific fields like medicine, environment, animals and plants